Composite

Part:BBa_K2757001:Design

Designed by: Bhuvana Sudarshan   Group: iGEM18_Oxford   (2018-10-10)


Art-175 fused with N-terminal DsbA 2-19 signal peptide and bidirectional pTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 86
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 534
    Illegal AgeI site found at 733
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Art-175 is synthesised by fusion of the SMAP-29 sheep myeloid peptide with the KZ144 endolysin originating from Pseudomonas aeruginosa. Bidirectional pTet promoter sequence taken from The application of Tet repressor in prokaryotic gene regulation and expression (Bertram and Hillen, 2007).

References

Bertram, R. and Hillen, W. (2007). The application of Tet repressor in prokaryotic gene regulation and expression. Microbial Biotechnology, 0(0), p.070824040706002